【摘 要】
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a pH-sensitive rhodamine-based probederivatives(RhM)was synthesized and explored as a fluorescence Off-On assay using Cu2+/RhM complex as a novel chemosensor for double channel detecting biothiols.The
【机 构】
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Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing,210009,China
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a pH-sensitive rhodamine-based probederivatives(RhM)was synthesized and explored as a fluorescence Off-On assay using Cu2+/RhM complex as a novel chemosensor for double channel detecting biothiols.The new small probe could bound to Cu2+reversibly and selectively under pH 3.0 to 7.0,which caused reversible change in the fluorescence intensity quenching and UV-visible absorbance restore.RhM was pH-sensitive which shows the highest fluorescence and absorbance response toward Cu2+ion under a pH 4.3 medium.Interestingly,biothiols such as GSH,Cys,Hcy could react with Cu2+/RhM complex resulting to release RhM reversibly over common amino acids,thereby enhancing the fluorescence and decreasing the absorbance.
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