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5. The Method of Attaining high transfection efficiency of plasmid into neural stem cells by electropor

The Method of Attaining high transfection efficiency of plasmid into neural stem cells by electropor

来源 :The 6th FAONS Congress and 11th Biennial Conference of CNS(第 | 被引量 : 0次 | 上传用户：njcdst

【摘 要】

：

Objective:To optimize the conditions of transfecting eukaryotic expression vector pEGFP-N1 into neural stem cells(NSCs)from rats by CUY21EDITII electroporator,in order to improve the transfection effi

【作 者】

：

Xiaohua Jiang Jingrui Tian Yanxia Tian Junling Gao 

【机 构】

：

School of Basic Medicine,Hebei Key Lab For Chronic Diseases,North China University of Science and Te

【出 处】

：

The 6th FAONS Congress and 11th Biennial Conference of CNS(第

【发表日期】

：

2015年3期

【关键词】

：

neural stem cells electroporation lipofectamine transfection 

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　　Objective:To optimize the conditions of transfecting eukaryotic expression vector pEGFP-N1 into neural stem cells(NSCs)from rats by CUY21EDITII electroporator,in order to improve the transfection efficiency of exogenous gene into NSCs and provide a basis for the follow-up experiments.

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