【摘 要】
：
The human neurotrophin-3(NT-3) DNA was obtained by RT-PCR from retinal tissue.Expression plasmid of NT-3, p EGFP-N1-NT-3 was constructed by inserting NT-3 DNA into plasmid p EGFP-N1.The p EGFP-N1-NT-3
【机 构】
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Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Labora
【出 处】
：
首都医科大学2013年研究生学术论坛
论文部分内容阅读
The human neurotrophin-3(NT-3) DNA was obtained by RT-PCR from retinal tissue.Expression plasmid of NT-3, p EGFP-N1-NT-3 was constructed by inserting NT-3 DNA into plasmid p EGFP-N1.The p EGFP-N1-NT-3 was transfected into 293T cells and the transfection efficiencies reached to 46.76% at 48 hours after transfection.
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