【摘 要】
：
Mammalian development begins with a single cell (zygote) resulted from the fertilization of a sperm and an oocyte.The zygotic genome undergoes profound epigenet
【机 构】
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InstituteofBiochemistryandCellBiology,ChineseAcademyofSciences,Shanghai
【出 处】
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The 11th transgenic Technology Meeting ISTT2013 ( 第十一届国际转基因技
论文部分内容阅读
Mammalian development begins with a single cell (zygote) resulted from the fertilization of a sperm and an oocyte.The zygotic genome undergoes profound epigenetic reprogramming to prepare for embryo development.The biological significance and mechanisms of epigenetic reprogramming are poorly understood.We find that 5-methylcytosine (5mC) is oxidized to 5-hydroxymethylcytosine (5hmC) as well as 5-carboxymethylcytosine (5caC) specifically in the paternal genomic DNA in zygotes.In vitro, Tet enzymes efficiently oxidize 5mC to 5caC under physiologically relevant conditions (e.g.in the presence of 1 mM ATP).
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