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利用随机扩增ＤＮＡ多态性ＲＡＰＤ方法，在美洲黑杨（Ｐ．ｄｅｌｔｏｉｄｅｓＭａｒｓｈ）×青杨（Ｐ．ｃａｔｈａｙａｎａＲｅｈｄ．）３代谱系中分析分子标记，构建出第１张美洲黑杨×青杨分子连锁图谱。共从３００个１０ｍｅｒ随机引物中筛选出７９个适合引物，检测出可供构图的分离标记１８０个。该图谱由２０个连锁群，１１０个ＲＡＰＤ标记组成。总图距为覆盖基因组总长度的７０３５％，标记间的平均间距为１７２７ｃＭ．连锁群长度在３７１～１８９８，相应标记分别在３～１０。本图谱为杨树抗病、虫和其它性状基因定位提供了框架结构，为实现杨树分子遗传育种迈进了最重要的一步。
Using random amplified polymorphic DNA (RAPD) method, molecular markers were analyzed in P.deltoidesMarsh × P.chayanaeRegeneration lineage 3 to construct the first linkage map of Populus × Poplar × Poplar Map. A total of 79 suitable primers were screened from 300 10mer random primers and 180 markers were obtained for patterning. The map consists of 20 linkage groups and 110 RAPD markers. The total map distance covers 70.35% of the total length of the genome and the average distance between markers is 17.27 cM. The length of the linkage group is 371 ~ 1898, corresponding markers are 3 ~ 10 respectively. The map provided a framework for the poplar disease resistance, gene mapping of insects and other traits, and took the most important step towards molecular poplar breeding of poplar.