Soft X-ray microscopy can provide a complementary spatial resolution between optical and electron microscopy,and to make full use of the high natural absorption contrast between organic materials and water in the photon energies which is between the K-absorption edge of oxygen(E ≈ 530 eV or λ = 2.34 nm)and carbon(E ≈ 280 eV or λ = 4.43 nm).The photon energies so called “water window” are especially suitable for imaging samples in aqueous media.Thus,hydrated biological specimens can be imaged in the water window photon energy range with no chemical fixation,dehydration,chemical staining and physical sectioning.Soft X-ray Nano-Computer Tomography can provide three-dimensional tomographic volumes to visualize subcellular structures with high resolution,up to 30 nm.Cryo-Soft X-ray microscopy had been used to visualize the internal architecture of fully hydrated cells [1-3] at high spatial resolution.