Objectives Small interfering RNA (siRNA) methodology suppresses specific gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis.siRNA technology is also a promising tool for gene therapy of various diseases.Lipofectamine RNAiMAX, a new transfection reagent, has been confirmed high efficiency in deliveringsiRNA into mesenchymal stem cells and coronary vascular smooth muscle cells.