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In the present work, the extraction of Re(Ⅶ) from hydrochloric acid solutions by OP-10/n-heptane/iso-amyl alcohol/N235/NaCl microemulsion was studied.The experiments were carried out using p-Octyl polyethylene glycol phenyl ether (OP-10) as surfactant and iso-amyl alcohol as co-surfactant.The mechanism in acidic condition was demonstrated by the log-log plot method.