Separation and purification of human tissue kallikrein and its biological functions

来源 :DaLian Internationall Symposia and Exhibition on Chromatogra | 被引量 : 0次 | 上传用户：xjp_djx

【摘要】

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【作者】

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Jennv J.Zhang Hailin Wang Maolan Xu Julie Chao Lee Chao Yukui Zhang

【机构】

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National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese A

【出处】

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DaLian Internationall Symposia and Exhibition on Chromatogra

【发表日期】

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2007年12期

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　　Human Tissue kallikrein (HTK), a 42 kDa serine protease, cleaves kininogen to generate bioactive peptide kinin, which binds to its receptors and triggers a wide spectrum of biological functions.Kallikrein is purified from urine by ion-exchange radial flow membrane chromatography and aprotinin-conjugated affinity chromatography.The separation condition was optimized.Purified kallikrein was confirmed by SDS-PAGE followed by comassie staining, matrix-assisted laser desorption: ionization mass spectrometery (MALDI), kllikrein activity assay and specific HTK ELISA.Biological functions of kallikrein have been studied in various animal models.

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Separation and purification of human tissue kallikrein and its biological functions

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