Bisphenol A(BPA),which is used as the primary raw material in the production of polycarbonate and epoxy resins,is a ubiquitous,endocrine-disrupting environmental contaminant.It could bind to nuclear receptors and interfere with synthesis,activity and degradation of hormone actions,so it may increase the incidence of hormone-dependent cancers1.As a bisphenol As substitute,Bisphenol S(BPS)is also shown to have estrogen effects.However,bisphenol substitutes(BPs)can be mainly metabolized to product BPs glucuronide and sulfate in vivo.And the genotoxicity and cytotoxicity of BPs and their metabolites are not reported by now.In this research,we used in vivo synthesis instead of traditional liver microsomes method.After gastric infusion of BPS,urine samples were collected from rats and separated by SPE.Then,BPS glucuronide was eluted with a 50%MeOH/H2O solution.The collected fraction was measured by LC-MS/MS.Then,the fraction was pooled and dried to 2.0 mL under a stream of nitrogen at 37 oC for further purification,and separated by semi-preparative HPLC.For BPS sulfate,we also developed a chemical method to improve its productivity 2,the solvent phenol was substituted by N,N-Dimethylformamide which could increase solubility of BPS and sulfur trioxide pyridine complex.In the next experiment,we will detect the biological functions of BPS metabolites in different cell lines.