【摘 要】
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Objective MicroRNAs comprise a family of small non-coding RNA molecules that have emerged as key post-transcriptional regulators of gene expression.Aberrant miRNA expression has been linked to various
【机 构】
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Cancer Center,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology
【出 处】
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中华放射学学术大会2016、中华医学会第23次全国放射学学术大会暨中华医学会第24次全国影像技术学术大会
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Objective MicroRNAs comprise a family of small non-coding RNA molecules that have emerged as key post-transcriptional regulators of gene expression.Aberrant miRNA expression has been linked to various human tumors.MicroRNA-330-3p has been reported to be up-regulated in NSCLC smaples and in tissues of NSCLC brain metastasis.However few studies have been focused on the relationship between MicroRNA-330-3p and neovascularization.Our study aimed to investigate the role of MicroRNA-330-3p for neovascularization after stereotactic body radiation therapy(SBRT)and explore the possible underlying mechanisms.Methods First,MicroRNA-330-3p overexpression was established by transfecting MicroRNA-330-3p precursor into non-small cell lung cancer H460 and HCC827 cells.Then Balb/c nude mice bearing H460 and HCC827 xenografts were treated with sham(control)and SBRT of 12 Gy in 1 fraction(12 Gy/1F).Tumor microvessel density(MVD)and function were examined at different times after irradiation.The activation of signaling pathways was determined using WB and RT-PCR analyses.Results Compared to the controls the MVD and hypoxia increased in the MicroRNA-330-3p overexpression tumors,which were accompanied by an decrease in the number of pericyte-covered vessels.Higher expression of HIF-1α,VEGFA and p-STAT3 were both found in protein and RNA levels in MicroRNA-330-3p overexpression tumors at different times after irradiation.Conclusion MicroRNA-330-3p appears to promote MVD as well as hypoxia,and repress the normalization of tumor vasculature after SBRT by increasing the expression of HIF-1α,VEGFA and p-STAT3..
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