The study of mechanism of precursor for brain-derived neurotrophic factor on hippocampal neuron surv

来源 :中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the | 被引量 : 0次 | 上传用户:tomily98
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Objective To investigate the intracellular mechanism of proBDNF on neuron by detecting expression of ELK-p,ERK and c-fos in cultured hippocampal neuron.Methods The hippocampal neurons were dissociated from El8 rats and cutured in neurobasal medium.Then the cells were treated with proBDNF,preBDNF and proBDNF antiserum,respectively.Thirty minutes,1 h or 48 h later,the cells were stained with Nissl solution and ELK-p,ERK2,c-fos immunocytochemistry were performed.Results It showed that the expression of ELK-p,ERK and c-fos was significantly upregulated in cultured hippocampal neuron after treated with proBDNF protein,and sometimes the immunoreactivity was seen in some nucleous.Whereas the expression was downregulated with proBDNF antiserum treatment,and many cells exhibited swelling and vasoculation.The immunoreactivity in preBDNF treated cells was similar to that in normal cultured cells.Conclusion It seems that proBDNF plays an important role in sustaining the hippocampal neuron survival through upregulating the ELK and ERK pathway.
其他文献
Homeostatic regulation of synaptic properties in response to chronic alteration of neuronal activity is essential for neural circuit function,but the underlying mechanism is largely unknown.Here we fo
Substance P (SP),the first identified neuropeptide,has long been indicated to be involved in the vagal control of heart rate and cardiac functions.However,the mechanism by which SP modulate cardiac va
Little is known about how gap junctions are involved in the respiratory-related,or any other types of,physiological neuronal activity since ongoing gap junction currents have never been characterized
In the primary visual cortex,activity of neurons evoked by stimuli within the classical receptive field (CRF) can be modulated by stimuli in the extra-receptive field (ERF).This modulating effect can
The spinal cord dorsal horn is the key site for transmission and modulation of nociceptive information via synaptic connections between primary nociceptive afferent C-fibers and secondary order sensor
Objective The immature and light-insensitive retina spontaneously generates a pattern of rhythmic bursting activity.Spatially,the activity is seen to spread across the retina in the form of waves and
Activity-dependent CREB-target gene transcription is implicated in cortical dendritic development.However,the molecular mechanisms regulating CREB-target gene transcription and dendritic morphogenesis
To explore the relationship between 5-HTergic terminals and the vestibulo-parabrachial projection neurons expressing 5-HT1A receptor (5-HT1AR) in the vestibular nuclear complex (VNC) of the rat.Tetram
Classic studies recognize two functionally segregated macroglial cell types in the central nervous system (CNS),namely astrocytes and oligodendrocytes.A third macroglial cell type including a unique p
Postsynaptic density-95 (PSD-95) is a scaffolding protein at excitatory synapses linking N-methyl-D-aspartate receptor (NMDAR) with neuronal nitric oxide synthase (nNOS),which contributes many physiol