Our current research focuses on development of a standalone,low-maintenance and sensitive detection system to monitor very small concentrations of chemical and biological contaminants in drinking water.The concept of evaporative concentration was proven by a single-line chip,as discussed in our previous report [1].Here we discuss the follow-up design,fabrication procedure and functional tests of a multiline evaporative sample concentrator integrated with a 3D printed polymer manifold platform.In contrast to previous work,20-fold enhancement of evaporation rate is demonstrated.The 16-lines device consists of a silicon-rich nitride (SiRN) membrane perforated with ~1.86 million micromachined via-holes (600 nm in radius),which forms the contact between a liquid channel and an overlapping evaporation chamber (Fig.1).The liquid evaporates through the grid of via-holes,and its evaporation rate is enhanced by effective renewal of saturated vapour layer at gas-liquid interface.A rapid prototyping technique (additive 3D printing) was applied to encapsulate silicon-glass chip within polymer manifold in semi-permanent manner.The adhesive pads were laser-cut to match the dimensions of microfluidic ports of silicon-glass evaporator (Fig.2).The temperature stabilization and monitoring of evaporation rate was provided by controller based on open-source Arduino electronics prototyping platform (Fig.3).The proportional-integral-derivative (PID) loop was software-implemented in 8-bit RISC microcontroller to stabilize the temperature with variation of 0.1℃.The communication with autonomous controller was facilitated via radio link established by single-chip 2.4 GHz transceivers and Bluetooth module.The chips were tested with respect to their evaporation capability.The influence of the gas flow on the evaporation rate was investigated for various sample flow rates (Fig.4).A linear relation between temperature and evaporation rate was observed for various sample flow rates.A very high water evaporation rates were achieved.An evaporation rate of 10 μL/min,achieved in mild conditions,compatible with biological sample treatment (T≤37℃),allowed for 4-fold sample enrichment (Fig.5).High-throughput flow ability and >30-fold concentration factor achieved in temperature of 50℃ facilitates the application of presented prototype also for chemical sample enrichment.