【摘 要】
：
目的：观察并比较复方汉防己和干扰素γ对肝星状细胞中转甲状腺素蛋白(transthyretin,TTR)、间-α胰蛋白酶抑制因子家族的重链之一(inter-α inhibitor H1,ITIH1)、α-2抗纤溶酶(serpin peptidase inhibitor clade F member 2,SERPINF2)表达的影响。方法：不同浓度复方汉防己和干扰素γ作用于肝星状细胞(Hepatic
【机 构】
：
中国人民解放军济南军区总医院,山东济南250031
【出 处】
：
第八届中国病理生理学会消化专业委员会学术会议
论文部分内容阅读
目的：观察并比较复方汉防己和干扰素γ对肝星状细胞中转甲状腺素蛋白(transthyretin,TTR)、间-α胰蛋白酶抑制因子家族的重链之一(inter-α inhibitor H1,ITIH1)、α-2抗纤溶酶(serpin peptidase inhibitor clade F member 2,SERPINF2)表达的影响。方法：不同浓度复方汉防己和干扰素γ作用于肝星状细胞(Hepatic Stellate Cell-T6,HSC-T6)细胞48h,流式细胞仪检测复方汉防己和干扰素γ对HSC增殖的影响,RT-PCR法检测TTR、ITIH1、SERPINF2在mRNA水平的表达,用ELISA检测上清液中TTR、ITIH1、SERPINF2 分泌,细胞免疫荧光检测HSC-T6细胞TTR、ITIH1、SERPINF2蛋白定位,Western blotting检测细胞中TTR、ITIH1蛋白的表达。
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