论文部分内容阅读
目的:探讨钙释放激活钙通道调节分子1(CRACM1,Orai1)对氧化型低密度脂蛋白(ox-LDL)诱导的人单核/巨噬细胞THP-1泡沫化的影响及其机制。方法:Western blotting方法检测蛋白表达,激光共聚焦显微镜观察Oria1 siRNA对THP-1结合、摄取DiI-LDL能力的影响。结果:80 mg/L ox-LDL可以时间依赖性地诱导Orai1表达,从12 h开始具有显著差异(P
OBJECTIVE: To investigate the effects of CaACM1 and Orai1 on ox-LDL induced human monocyte / macrophage THP-1 foam and its mechanism. Methods: Western blotting was used to detect the protein expression. The effect of Oria1 siRNA on the binding of THP-1 to DiI-LDL was observed with laser confocal microscopy. RESULTS: Orai1 expression was induced by 80 mg / L ox-LDL in a time-dependent manner, with a significant difference at 12 h (P