【摘 要】
：
In proteome studies, proteolytical digestion is a necessary step for the identification of proteins by mass spectrometry (MS).At present, the prevalent meth
【机 构】
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Department of Bioteclnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dali
【出 处】
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DaLian Internationall Symposia and Exhibition on Chromatogra
论文部分内容阅读
In proteome studies, proteolytical digestion is a necessary step for the identification of proteins by mass spectrometry (MS).At present, the prevalent methods for protein digestion are performed in solution, which suffered the disadvantages of low digestion efficiency, autodigestion, instability toward chemical denaturants and organic solvents, and recycle unavailable.To achieve the fast identification of proteins, immobilized trypsin has been utilized under various configurations, among with the immobilized trypsin microreactors show the virtues of high digestion efficiency, low autodigestion, good stability, easy recycle, etc.
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