Since April 2010,domesticated ducks in China have been suffering from an emerging infectious disease characterized by retarded growth,high fever,loss of appetite,decline in egg production,and death.
为同时在昆虫细胞中按照合适比例表达禽腺联病毒的三个结构蛋白,首先根据禽腺联病毒VP 基因序列设计引物,扩增出VP 基因,将其克隆至杆状病毒表达载体pFastBac1,获得重组转移载体pFastBac-VP, 将其转化到DH10Bac 感受态细胞中, 经抗性和蓝白斑筛选, 获得重组穿梭质粒rBacmid-VP,在脂质体的介导下转染昆虫细胞Sf9,获得重组杆状病毒rBac-VP.
Glycoprotein C is one of the duck plague virus (DPV) glycoproteins and is encoded by the DPV UL44 gene.
鸭瘟病毒(DEV)是一种α 疱疹病毒,能引起鸭、鹅、天鹅等雁形目水禽产生一种急性、热性、败血性、接触性传染病。防治该病的主要措施是定期接种鸭瘟疫苗,常用的疫苗为鸭瘟病毒减毒活疫苗。
Duck plague is an acute,contagious disease of ducks,geese,and swans that is caused by an alphaherpesvirus [duck plague virus (DPV); Anatid herpesvirus 1].
本研究旨在建立一种可检测3 型鸭甲型肝炎病毒(DHAV-3)的反转录-环介导等温扩增方法(RT-LAMP)。根据DHAV-3 基因的保守序列,设计一套RT-LAMP 引物,以样品的cDNA 为模板,利用Bst DNA 聚合酶,在63℃恒温条件下进行扩增,扩增产物中加入钙黄绿素和氯化锰染色直接或在紫外光下观察判定扩增结果。
Avian hepatitis E virus(HEV)is related genetically and antigenically to swine HEV and their capsid protein share common epitopes.
禽戊型肝炎病毒(Hepatitis E virus,HEV)是2001 年发现的导致鸡大肝大脾病或肝炎脾大综合症的主要病原,主要引起30-72 周龄的蛋鸡和种鸡的死淘率升高(1-4%)和产蛋率下降(20-40%)。
A reverse transcription loop-mediated isothermal amplification(RT-LAMP)was developed for the detection of duck hepatitis virus type-1(DHV-1).