Aim:To investigate the effect of lentivirus mediated Integrin-linked kinase (ILK) RNA interference (RNAi) on human retinal Müller cells transdifferentiation into contractile myofibroblasts.Methods:Lentiviral vector expressing ILK-specific shRNA was constructed and introduced into cultured retinal Müller cells,the silencing of ILK gene was identified by real time RT-PCR and western blot methods; Müller cell phenotype change was confirmed by immunodetection of-SMA stress fiber formation; Generation of tractional force was assessed using a tissue culture assay with cells incubated in three-dimensional collagen gels; cell migration was determined by the Boyden chamber method,using 10％ fetal calf serum (FCS) as a chemotactic factor.