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5%-25% of HIV-1 infected individuals produce broad neutralizing antibodies (bNAb).A HIV-1 vaccine (RV144) has shown a protection partially associated with epitopes within envelope V2 regions.It is well accepted that both NAbs and non-NAbs are important for protection against HIV infection.However, the mechanisms for antibody production in HIV-1 vaccination are still less known.In the present study, to develop HIV-1 envelope immunogens and explore T follicular helper function in Ab production, we modified the envelope derived from a HIV-1 primary R5 strain, immunized mice using DNA prime-protein boost strategies.Antibody production, T cell development, TFH responses as well as B cell differentiation by the vaccination have been examined.