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The cementation proces fors bio-cement binding loose sand particles is researched by X-ray Computed Tomography(X-CT),compressive strength,porosity and calcite content respectively.The structures of 2D cross-sections at different positions in the same bio-sandstone sample for 0 day,3 days and 7 days are characterized by X-CT and the 3D reconstruction of the same sample are also investigated by X-CT.