【摘 要】
：
Arbuscular mycorrhizal fungi(AMF)have great potential for assisting metalhyperaccumulating plants in the remediation of contaminated soils.However,little information is available about the symbiosis a
【机 构】
：
State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese ResearchAcademy of Enviro
【出 处】
：
The 17th International Conference on Heavy Metals in the Env
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Arbuscular mycorrhizal fungi(AMF)have great potential for assisting metalhyperaccumulating plants in the remediation of contaminated soils.However,little information is available about the symbiosis and community composition of AMF associated with manganese(Mn)hyperaccumulator,such as Phytolacca americana,growing on Mn-contaminated soils under natural conditions.Therefore,the objective of this study was to analyze AMF diversity and community composition in P.americana roots growing at an Mn mining site.Molecular techniques were used to analyze AMF community composition and phylogenetic relationship in P.americana roots sampled from three Mn mine spoils and one adjacent reference areas.Results obtained showed that mycorrhizal symbionts successfully established even in the most heavily Mn-polluted sites.Root colonization and AMF diversity were significantly negatively correlated with total and extractable Mn concentrations.Principal component analysis(PCA)revealed that Mn contamination impacted AMF diversity,and shaped AMF community structure.Phylogenetic analyses demonstrated that all species were affiliated with Glomus,suggesting that Glomus was the dominant genus in this AMF community.Some unique sequences that occurred exclusively in heavily polluted sites associated with P.americana ay belong to symbiotic fungi with great potential for improving the phytoremediation efficiency of Mn-contaminated soils.
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