In this paper,a new biological cross-linker,microbial transglutaminase (mTGase) was used to catalyze the immobilization of lactoferrin onto the wool fabrics and the antibacterial properties of wool immobilizing lactoferrin,which shows antimicrobial activity on both Gram-negative and Gram-positive bacteria,were determined.It was found that the minimal inhibitory concentration (MIC) of lactoferrin against S·aureus and E·coli is 0.5mg/mL and 0.25mg/mL.Compared with the control sample,the amount of lactoferrin adhered onto the wool fabric was improved from 4.87 mg·(g fabric)-l to 12.96 mg(g fabric)-1.That means the crosslinking reaction initiated by mTGase can mcrease the amount of lactoferrin fixed onto wool fabric obviously.The ratios of bacteriostasis to S.aureus and E.coli of wool fabrics immobilizing lactoferrin reached 57.95% and 69.96%,respectively,showing good antibacterial property.