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In the present study,the performance of ATB Fungus 3 (bioMérieux,France),with the endpoints read visually and automatically, were evaluated for testing the antifungal susceptibility of Candida spp.to fluconazole,voriconazole,itraconazole and amphotericin B.A collection of 218 Candida isolates from CHIF-NET10 were test ed,including 81 isolates of Candida albicans,42 of Candida glabrata sensu stricoto, 41 of Candida tropicalis, 38of Candida parapsilosis complex and 16 of Candida krusei.Broth microdilution(BMD) method was used asgold standard following CLSI instructions.The results were considered to be in essential agreement(EA) when the test results were within two dilutions of the reference value.