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目的:通过检测脑组织内丙二醛(MDA)、超氧化物歧化酶(SOD)含量和谷胱甘肽过氧化物酶(GSH-Px)活性,探讨亚低温、川芎嗪对缺氧缺血性脑病幼鼠的脑保护作用。方法:将142只大鼠幼鼠随机分为常温缺血组(A组)、常温缺血+亚低温组(B组)、常温缺血+川芎嗪组(C组)、常温缺血+亚低温+川芎嗪组(D组),建立缺氧缺血性脑病幼鼠模型,各组给予相应的治疗,检测各组幼鼠脑组织内MDA、SOD含量及GSH-Px活性,对结果进行比较和统计分析。结果:B组、C组、D组幼鼠MDA含量均较A组低,SOD含量及GSH-Px活性高于A组,提示亚低温和川芎嗪治疗缺氧缺血性脑病有效;D组脑组织内MDA含量较B组、C组低,SOD含量、GSH-Px活性较B组、C组高,差异有统计学意义(P均<0.01)。B、C两组MDA和SOD含量及GSH-Px活性比较差异无统计学意义(P>0.05)。结论:亚低温、川芎嗪均能减轻缺氧缺血引起的脑损伤,二者联用疗效更佳。
OBJECTIVE: To investigate the effects of mild hypothermia, ligustrazine on hypoxia and ischemia (Hypoxic-ischemic-reperfusion injury) by detecting the content of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) Cerebral protection in encephalopathy young rats. Methods: 142 male rats were randomly divided into four groups: normal temperature ischemia group (A group), normal temperature ischemia + hypothermia group (B group), normal temperature ischemia + ligustrazine group (C group) The rats in hypoxia-ischemic encephalopathy model were established by hypothermia + ligustrazine group (D group). The rats in each group were given the appropriate treatment. The content of MDA and SOD and the activity of GSH-Px in brain of each group were detected, and the results were compared And statistical analysis. Results: The contents of MDA in group B, group C and group D were lower than those in group A, and the content of SOD and the activity of GSH-Px in group A were higher than that in group A, which suggested that hypothermia and ligustrazine were effective in treating hypoxic-ischemic encephalopathy. The content of MDA in the tissue was lower than that in the B group and the C group, and the activity of SOD and the content of GSH-Px in the tissue were higher than those in the B and C groups (P <0.01). There was no significant difference in the content of MDA and SOD and the activity of GSH-Px between B and C groups (P> 0.05). Conclusion: Mild hypothermia, tetramethylpyrazine can reduce the hypoxic-ischemic brain damage caused by the combination of both better.