To study the anti-HBV activity of homoharringtonine、 IFN-a 1b 、 hydroxycamptothecin and norfloxacin invitro.The four drugs were respectively added to HepG2.2.15 cell line transfected with HBVDNA.After 8 days, Cytopathic effect (CPE) was observed.Under the maximum non-toxic conentration(TC0), ELISA and Fluorescent quantified PCR were used for determining HBsAg、 HBeAg and HBVDNA in cell culture medium.The resulets show that homoharringtonine、 IFN-α 1b and hydroxycamptothecin have all apparent inhibition effect to HBV in vitro.Inhibition effect do not be seen in the other concentration of norfloxacin.This will provide some certain experience basis for the therapy of clinical anti-HBV.