摩拉水牛和尼里/拉菲水牛PRKAA2基因第四外显子的SNPs检测

来源 :2017第四届中国肉牛选育改良与产业发展国际研讨会 | 被引量 : 0次 | 上传用户：finney_young

【摘要】

：

为研究水牛PRKAA2基因第四外显子多态性,本研究以广西水牛研究所的摩拉水牛和尼里拉菲水牛为材料,扩增其PRKAA2基因第四外显子,并通过常规测序法检测其SNPs.经测序发现,在实验所扩增的目的片段内包含有PRKAA2第三内含子部分序列,且在此部分序列内存在3个SNP位点(IVS3.557T＞C,IVS3.560C＞T,IVS3.565G＞A).而在PRKAA2第四外显子内存在1个SNP位点(c.

【作者】

：

黄玥萌郑海英杨春艳黄加祥鄢胜飞李舒露于农淇李孟琪尚江华

【机构】

：

中国农业科学院广西水牛研究所,农业部(广西)水牛遗传繁育重点实验室,广西南宁530001

【出处】

：

2017第四届中国肉牛选育改良与产业发展国际研讨会

【发表日期】

：

2017年3期

【关键词】

：

水牛 PRKAA2基因第四外显子遗传多态性

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

其他文献

Fatty acid composition in different muscle depot during growth of Yanbian Yellow Cattle

We hypothesized that fatty acid composition would differ among different muscle depots andover time on a finishing diet.The present study was conducted with 16 Yanbian Yellow Cattle steers (~ 8 mo of

会议

Beef cattleAgeMuscleFatty acidGene expression

Multi-strategy genome-wide association studies identify the DCAF16-NCAPG region as a susceptibility

Average daily gain (ADG) is the most economically important trait in beef cattle industry.Using genome-wide association study (GWAS) approaches,previous studies have identified several causal variants

会议

ADGGWASSNP

Comparative Transcriptome Analysis druing skeletal muscle postnatal development in Bovines of Differ

Gender and slaughter age are two key pre-slaughter variables that can significantly affect the skeletal muscle development and the beef palatability,but its mechanims is still not well kown.Here,total

会议

BovinesTranscriptome analysisSkeletal muscle postnatal developmentSexesAges

Effects on the eating quality of beef used Chinese processing methods

This study was to evaluate the effects of several Chinese processing methods by steaming,boiling,braising,pan-frying,frying,roasting,drying on eating quality of beef.The shear force,color and TPA (har

会议

BeefProcessing methodsEating qualityTPA

Longissimus Dorsi Muscle Transcriptomic Analysis of Yunling and Chinese Simmental Cattle Differing i

Intramuscular fat (IMF) content and fatty acid (FA) composition vary significantly across beef cattle breeds,which play important roles in taste and nutritional value.However,the molecular mechanisms

会议

Beef cattlelongissimus dorsi muscletranscriptomeintramuscular fat contentfat

A CNV-associated QTL in GBP Cluster Affects Adult Cattle Stature Revealed by Meta-QTL and Overview A

会议

Evaluation and regulation of intracellular cysteine protease to improve the quality and quantity of

Over these five decades,livestock production (breeding) methods have been rapidly developed from the both sides of male and female animals as well as contribution of genetic improvement.In particular,

会议

CattleOocyteEmbryoCathepsinE64PyridoxineDevelopmental competenceApoptosis

Requirement of MEF2A in bovine skeletal muscle primary myoblast differentiation

The proliferation and differentiation of skeletal myoblasts are important in controlling muscle growth and improving livestock muscle quality.MEF2A is an important transcription factor in regulating s

会议

bovineMEF2Amyoblastdifferentiation

秦川肉牛CGI-58基因5'-调控区一个新SNP与肉质的关系及对启动子活性的影响

探究秦川肉牛CGI-58基因5-调控区中一个新单核苷酸多态位点(Single nucleotide polymorphism,SNP)与肉品质的关联性及对启动子活性的影响.PCR扩增CGI-58基因5-调控区,通过直接测序技术检测其单核苷酸多态性,并使用SAS V8提供的一般线性模型(GLM)对单核苷酸多态位点和肉质性状进行关联分析.构建含有SNP位点不同基因型的双荧光素酶报告载体然后转染3T3L

会议

肉牛CGI-58基因5'-调控区单核苷酸多态位点启动子活性肉品质

宣汉牛微卫星DNA与生长发育性状的关联分析

旨在分析微卫星DNA与宣汉牛长发育性状的关联性,为宣汉牛品种资源保护和选育改良提供理论依据.利用12对微卫星引物,采用PCR扩增和非变性聚丙稀酰胺凝胶电泳技术,对42头宣汉牛母牛进行了遗传多样性检测,并统计分析各位点不同基因型群体宣汉牛在体高、体长、胸围、管围和体质量5个性状上的效应关系.结果显示,12个微卫星位点中,BM1905位点与宣汉牛体高、体长、胸围、管围和体质量不具有关联效应.IDVGA

会议

黄牛微卫星DNA遗传多样性生长发育

摩拉水牛和尼里/拉菲水牛PRKAA2基因第四外显子的SNPs检测

与本文相关的学术论文