施氏假单胞菌F4是一株能高效生物降解黄曲霉毒素B1(Aflatoxin B1,AFB1)的菌株.为了了解F4生物降解AFB1的机理,采用HPLC对经二氯甲烷萃取分离的降解液进行柱分离,并利用飞行时间质谱(Q-TOF)对降解产物可能的结构进行分析.
本文通过硫酸铵分级沉淀、DEAE-Sepharose阴离子层析和Superdex G-100凝胶排阻层析,从施氏假单胞菌(Pseudomonas stutzeri)F4中分离纯化黄曲霉毒素B1降解酶(AFB1-degrading enzyme,ADE).
A novel NhaD-type Na+/H+ antiporter gene designated Hb_nhaD was cloned from the halophilic bacteria from Daban Salt Lake by using Escherichia coli Knabc lacking three major Na+/H+ antiporters.
Double-stranded RNA (dsRNA) regulated protein kinase (PKR) is a key regulator involved in multiple signaling pathways.Our study aimed to identify more PKR interacting proteins through tandem affinity
Bacteria transport numerous substrates across cellular membranes via secretion systems that are essential for virulence and survival.Seven general classes of secretion systems, termed type Ⅰ to type Ⅶ
对分离自庐山土样的放线菌JXJ-0135溶藻活性成分的发酵时间、溶藻活性、溶藻范围、对其他水生生物的毒性、溶藻作用机理和活性成分的结构进行了研究.
百草枯(PQ)在水稻田中使用频繁,半衰期长,造成的环境污染已不容忽视.水稻田在种植过程中因覆水而经常处于厌氧状态,因此本文构建厌氧反应体系,研究铁/腐殖质还原菌、腐殖质对PQ降解速率的影响,并研究PQ降解与腐殖质模式物蒽醌-2,6-二磺酸盐(AQDS)还原的相互作用过程,初步探讨PQ在这一体系中的降解机制和PQ降解促进途径.
从庐山土样中筛选到一株具有肥料效应的放线菌JXJ-0136,16S rRNA基因序列发育分析表明,该菌是链霉菌属的成员,与浅紫链霉菌(Streptomyces violascens)的亲缘关系最近,相似性为97.98%.