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5. AFSM sequencing approach a simple and rapid method for genome-wide SNP and methylation site discover

AFSM sequencing approach a simple and rapid method for genome-wide SNP and methylation site discover

来源 :第十六届全国植物基因组学大会 | 被引量 : 0次 | 上传用户：GISSeven

【摘 要】

：

　　We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular

【作 者】

：

Zhiqiang Xia Meiling Zou Shengkui Zhang Binxiao Feng Wenquan Wang 

【机 构】

：

TheInstituteofTropicalBiosciencesandBiotechnology,ChineseAcademyofTropicalAgricultureSciences,Haikou

【出 处】

：

第十六届全国植物基因组学大会

【发表日期】

：

2015年8期

【关键词】

：

single nucleotide polymorphisms (SNP) cassava amplified fragment SNP and methyla 

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论文部分内容阅读

　　We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular marker-assisted breeding strategy based on the use of two restriction enzyme pairs (EcoRI-MspI and EcoRI-HpaⅡ) and a next-generation sequencing platform.

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