MYB 转录因子是植物中最大类的转录因子家族之一,广泛参与植物生长发育过程,如细胞形态的建成和分化、植物生长中的信号转导、次级代谢的调控、生物和非生物胁迫的应答等。本论文从人参发根转录组测序结果中筛选到一个MYB 基因PgMYB2,通过构建pCambia1302-PgMYB2 过表达载体,在洋葱中定位显示PgMYB2 定位于细胞核中。
the formation of new blood vessels by endothelial cells from existing microvessels,is a critical process in solid tumor.Accordingly,the inhibition of angiogenesis is considered to be a promising strat
WRKY proteins belonging to a large transcription factor(TF)family are found solely in plants.They contain either one or two domains,a 60 amino acids region defined by a highly conserved WRKYGQK motif
The v-myb avian myeloblastosis viral oncogene homolog(MYB)family constitutes one of the most abundant groups of transcription factors and plays vital roles in developmental processes and defense respo
牻牛儿基焦磷酸合成酶(GPPS,Geranyl diphosphate synthase)是单萜化合物合成的关键酶,催化1 分子的IPP 与DMAPP 形成GPP,为单萜化合物的合成提供碳骨架。根据川西獐牙菜(Swertia mussoti)转录组SmGPPS 基因序列,设计特异性引物,通过RT-PCR 扩增得到cDNA 序列,并进行TA 克隆、测序及序列分析;构建原核表达载体pET28a-SmG
A set of bi-templates molecularly imprinted polymers(BT-MIPs)were prepared by using quercetin-rutin mixture as co-templates in present study.The ratio of total volume of templates/functional monomer/c
人参(Panax ginseng C.A.Meyer)是多年生五加科草本药用植物,具有极高的药用价值,人参皂苷(ginsenoside)为其主要药用成分.人参皂苷的积累受合成酶和转运蛋白等相关基因高度调节,这些基因的表达受各种转录因子(transcription factor,TF)的高度调控.