【摘 要】
：
耐药肺结核病特别是耐多药肺结核病(MDR-TB)的流行对当今肺结核病的有效治疗提出了重大挑战.本研究通过iTRAQ-2DLC-MS/MS和Solexa测序技术筛选了MDR-TB、非耐药肺结核病(DS-TB)和正常对照间蛋白组学和转录组学的差异,发现在MDR-TB组有50个蛋白质以及43个差异miRNAs与DS-TB组和正常对照组组均存在差异.进一步靶基因预测和整合分析,发现22.00％的差异蛋白质
【机 构】
：
浙江大学细胞生物学研究所,杭州310058 绍兴市第六人民医院,绍兴312000
【出 处】
：
2015中国转化医学大会暨中国转化医学联盟成立大会
论文部分内容阅读
耐药肺结核病特别是耐多药肺结核病(MDR-TB)的流行对当今肺结核病的有效治疗提出了重大挑战.本研究通过iTRAQ-2DLC-MS/MS和Solexa测序技术筛选了MDR-TB、非耐药肺结核病(DS-TB)和正常对照间蛋白组学和转录组学的差异,发现在MDR-TB组有50个蛋白质以及43个差异miRNAs与DS-TB组和正常对照组组均存在差异.进一步靶基因预测和整合分析,发现22.00％的差异蛋白质和32.56％的差异miRNAs间能够建立渊控关系网络,且多存在于补体和凝血途径,从中我们选取了有对应凋控关系的3对蛋白质-miRNAs： CD44antigen(CD44)-miR-4433b-5p,Coagulation factor Ⅺ (F11)-miR-424-5p,和kininogen-1(KNG1)-miR-199b-5p进行验证.验证实验发现KNGl和miR-424-5p在MDR-TB、DS-TB和正常对照3组间均存在显著差异,并且KNGl与miR-199b-5p存在负相关(r=-0.232, P=0.017).F11和miR-4433b-5p只在MDR-TB和DS-TB间有差异,CD44和miR-199b-5p只在MDR-TB和正常对照间有差异.我们最终确立了以CD44,KNGl,miR-4433b-5p,miR-424-5p,miR-199b-5p为节点的MDR-TB诊断模型.我们的研究为MDR-TB提供了一组新的诊断标志物,为阐明机体蛋白组学和转录组学标志物在MDR-TB发病中的作用提供了新的实验依据.
其他文献
Background: Pulmonary tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis (Mtb) infection.The infectious process of Mtb brings various pathological changes such as i
AIM:Long non-coding RNAs (lncRNAs) are transcripts with length greater than 200 nucleotides and without protein-coding function.They are emerging as new regulators in cancer biology by acting as turno
背景与目的：儿童青少年肥胖的发生是遗传因素和环境因素共同作用的结果。近些年,以单核苷酸多态位点(Single Nucleotide Polymorphism,SNP)和拷贝数变异(Copy Number Variation, CNV)为标记的遗传易感性分析在欧裔人群得到了许多与肥胖发生风险相关的位点和区域。但这些标记在我国儿童青少年中的研究较少,且鲜见有关遗传因素与环境因素(如饮食行为)的交互效应
Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens.The present study aimed to investigate
Diabetes mellitus (DM) is a component of metabolic syndrome characterized by hyperglycemia and insulin resistance.Urine testing for sugar was commonly used as a method of clinical detection to support
There was lack of a rapid and efficient determination method for cured pulmonary tuberculosis (TB).iTRAQ labeling coupled with 2D LC-MS/MS analysis was used to identify the differentially expressed se
BACKGROUND: It has been shown that circular RNA (circRNA) is associated with human cancers, however, few studies have been reported in hepatocellular carcinoma (HCC).OBJECTIVE: To estimate clinical va
目的 探讨血清肿瘤标志物神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段(CYFRA21-1)、鳞状上皮细胞癌抗原(SCC-Ag)、癌胚抗原(CEA)及糖类抗原125(CA125)联合检测在肺癌诊断及分型中的价值.方法 用化学免疫发光法检测107例肺癌患者(鳞癌46例、腺癌38例、小细胞肺癌23例)、53例肺部良性疾病患者及45例健康人血清NSE、CYFRA21-1、SCC-Ag、CEA及CA1
Backgrounds and objectives: The importance of genes in congenital and infantile steroid resistant nephrotic syndrome(SRNS) are well established and accepted,however, screening for children (1-13years)
目的：探讨肝癌组织来源的血浆miRNA在肝细胞肝癌(HCC)患者中的表达并评估血浆miRNAs作为HCC早期诊断标志物的价值.方法：采用三阶段研究设计.第一阶段，在HCC患者肝癌移植术或肝癌切除术前及术后的血浆样本和肝癌患者的癌组织、癌旁组织及肝硬化患者的肝硬化组织中通过TLDA芯片系统筛选差异表达的miRNA.第二阶段，采用病例对照研究设计在大样本HBV阳性HCC患者和HBV携带者中验证第一阶段