【摘 要】
:
The utility of RNA-Sequencing and the broad range of organisms to which it is applied require analysis tools that can be effectively leveraged across divers
【机 构】
:
KlarmanCellObservatoryattheBroadInstituteofMassachusettsInstituteofTechnology
【出 处】
:
第一届广州核酸国际论坛:新一代测序、生物信息学与非编码RNA
论文部分内容阅读
The utility of RNA-Sequencing and the broad range of organisms to which it is applied require analysis tools that can be effectively leveraged across diverse realms of biology.Existing reference genomes or draft genome assemblies may yield a limited view of the transcript content of a sample due genome misassembly, sequencing gaps, or genuine sequence variation between sequenced samples and available reference genomes.Here we present a hybrid approach that combines genome-guided and de novo RNA-Seq assembly to annotate gene structures and capture those transcripts missing or otherwise insufficiently represented by genome assemblies.We demonstrate our approach to be highly effective across diverse eukaryotes and to the restructured genomes of human breast cancer cell lines.
其他文献
目的:探讨碱性水解三偏磷酸纳(sodium trimetaphosphate,STMP)与Ⅰ型胶原分子间的吸附行为及在仿生再矿化中的作用机制.方法:收集人第三磨牙制备矿化牙本质粉末,蚁酸脱矿后
结肠癌是常见的发生于结肠部位的消化道恶性肿瘤,好发于直肠与乙状结肠交界处.在所有癌症类型中,结肠癌(colon cancer)的发病与膳食营养的关系最为密切.结肠癌在西方国家的发
目的:通过对常见颌面畸形的手术设计方案、治疗方法及手术治疗效果的分析、总结,寻求治疗此类患者的合理治疗途径,为临床治疗同类患者提供参考.方法:对1999年7月至2012年1月
目的:通过分析发作性睡病与癫痫共患的临床诊断及治疗过程,提高对此类共患病例的认识.方法:分析2例患儿临床资料、视频脑电图监测(VEEG)及多次睡眠潜伏期试验(MSLT)结果,其
目的:探讨利用Medpor假体对鼻背、鼻基底填充或进行鼻中隔延长,结合鼻中隔、耳廓软骨鼻尖部植入综合鼻整形的手术方法.方法:取鼻小柱正中蝶形切口加双侧鼻翼缘切口行鼻背Medp
The Traditional BiomarkerSerum creatinine当GFR下降50%以上时,Scr才开始上升主要反应GFR—肾小球滤过功能肌肉的代谢产物,蛋白类食物在体内代谢后产生外源性Cr.Cr不在体内
目的:了解东西方鼻形态与软骨、骨及肌肉皮肤之间的关系;以期望从组织学和动力学角度使鼻整形效果趋于完美.方法6例亚洲人与6例美国人鼻初次整形时,对鼻头皮肤、皮下组织,鼻
C.elegans Argonaute-small-RNA pathways function in epigenetic inheritance.For example, the PIWI Argonaute PRG-1 initiates RNA-induced epigenetic silencing (
The dynamic usage of mRNA 3 untranslated region (3UTR) resulting from alternative polyadenylation (APA) is emerging as a pervasive mechanism to regulate app
Protein synthesis is quantitatively determined by translation initiation and elongation.However, these two aspects have not been decoupled at genomewide sca