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In this study, characterization of ARAhDRRP gene (gb|DQ296045.1), a new member of the peanut defensin family and its potent anti-Aspergillus flavus invasion activity in peanut were analyzed.The results showed that the AhDRRP gene was 585 bp in full-length with an 255 bp open reading frame, it encoded a 75-amino-acid polypeptide, and showed 33%-70% homology with other plant species.