【摘 要】
：
To solve the problem of the donors on the breakage and damage of aptamers LRET-based optical biosensor of an aptamer-upconversion conjugate was constructed base on the advantages of aptamer used for b
【机 构】
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State Key Laboratory of Luminescence and Applications,Changchun Institute of Optics,Fine Mechanics a
【出 处】
：
首届国际微纳米体系发光及光电性质学术会议
论文部分内容阅读
To solve the problem of the donors on the breakage and damage of aptamers LRET-based optical biosensor of an aptamer-upconversion conjugate was constructed base on the advantages of aptamer used for biodetection and the upconversion nanoparticles,which can perform infrared-to-visible photon conversion in the doped rare-earth ions.The research included: finding an effective way to improving the efficiency of upconverison luminescence; studing on the method,by which the nanoparticles could be bonded with avidin-biotin at 5end of aptamer in order to keep their the high sensitivity and specificity; invesgating the energy transfer and dynamic process between UCNPs labelled aptamer and the quenching fluorescence TAMRA; finally evaluating the stability,sensitivity and specificity of the as-designed biosensor.This biophotonswitch has promising potential for applications in broad areas,such as homogeneous bioassay,liquid biochip or liquid array for bioassay and in situ imaging,where reusability and excellent photostability as provided by this biosensor,are greatly requested.
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