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目的:建立布渣叶提取物中牡荆苷、异牡荆苷和水仙苷的含量测定方法。方法:采用Waters XbridgeTMC18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸水溶液,二元梯度洗脱,流速0.8 mL.min-1,检测波长320 nm,柱温25℃。结果:牡荆苷、异牡荆苷和水仙苷分别在8.512～29.79,8.208～28.73,41.60～145.6 mg.L-1与峰面积呈良好的线性关系,平均加样回收率分别为96.30%,97.32%,102.22%。结论:方法简便、可行,重复性好,结果准确,可作为布渣叶提取物的含量测定方法,为其质量控制提供参考依据。
Objective: To establish a method for the determination of vitexin, isovitexin and narcissus in cloth residue extract. Methods: A Waters Xbridge TMC18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of methanol-0.1% phosphoric acid solution with a binary gradient elution at a flow rate of 0.8 mL.min-1. . Results: Vitexin, isovitexin and narcissus had good linearity between 8.512 ~ 29.79, 8.208 ~ 28.73 and 41.60 ~ 145.6 mg.L-1, respectively. The average recoveries were 96.30% 97.32%, 102.22%. Conclusion: The method is simple, feasible, reproducible and accurate. It can be used as a method for the determination of the content of Cloth Residues extract and provide a reference for its quality control.