【摘 要】
：
本文就芸薹黄化病毒与PEMV-2协生互作进行了研究，通过农杆菌浸润接种的方法使BrYV与PEMV-2共同侵染本生烟，18℃下接种两局后BrYV与PEMV-2在本生烟上能产生协生症状，表现为新生叶先发病，叶片向下卷曲，在叶片上产生大片褪绿斑，之后褪绿斑逐渐变成坏死斑，而BrYV或PEMV-2单独侵染本生烟均不产生明显症状。24℃下，BrYV与PEMV-2也能产生协生症状，但与18℃相比，协生症状明显
【机 构】
：
中国农业大学植物病理学系/农业生物技术国家重点实验室,北京 100193
【出 处】
：
中国植物病理学会2014年学术年会
论文部分内容阅读
本文就芸薹黄化病毒与PEMV-2协生互作进行了研究，通过农杆菌浸润接种的方法使BrYV与PEMV-2共同侵染本生烟，18℃下接种两局后BrYV与PEMV-2在本生烟上能产生协生症状，表现为新生叶先发病，叶片向下卷曲，在叶片上产生大片褪绿斑，之后褪绿斑逐渐变成坏死斑，而BrYV或PEMV-2单独侵染本生烟均不产生明显症状。24℃下，BrYV与PEMV-2也能产生协生症状，但与18℃相比，协生症状明显减弱，通过重组马铃薯x病毒(PVX)分别表达PEMV-2的开放阅读框3(ORF3)和ORF4能够增加BrYV在本生烟的病毒积累量，表明PEMV-20RF3编码的长距离运动蛋白与ORF4编码的胞间运动蛋白都参与两病毒的协生互作。
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