Protein elicitor PeaT1, which was originally isolated from Alternaria tenuissima, could induce systemic acquired resistance (SAR) to tobacco mosaic virus (TMV) in tobacco plants.In our study, a 22 kDa tobacco plasma membrane protein which could interact with PeaT1 was isolated via Far Western Blot (GenBank: CAA65195.1) and it was designated as PREN (Potential Receptor for an Elicitor with the NAC-domain).The MS analysis for the PREN indicated it belonging to DREPP (developmentally regulated plasma membrane polypeptide) family, which was involved in plant stress responses and signaling transduction.Far-Western blot and Co-Immunoprecipitation analysis showed that PREN could associate with PeaT1 in vitro under denatured and native conditions, respectively.Bimolecular fluorescence complementation (BiFC) data indicated that PeaT1 could associate with PREN in living tobacco cells.VIGS assay result showed that PREN silenced tobacco was more susceptible to TMV than TRV control when treated with PeaT1, providing convincing evidence that PREN could perceive the stimulus from PeaT1 which induced SAR in tobacco.While downstream signaling pathway needs further study, one thing for certain is that PREN plays an important role as a transducer to perceive the stimulus from PeaT1 and mediate the downstream resistance signaling in tobacco plants.