【摘 要】
:
The genetic variations in three mitochondrial DNA(mtDNA)regions,namely portion of cytochrome c oxidase subunit 1(pcox1),NADH dehydrogenase subunit 1(pnad1)and cytochrome b(pcytb),were examined for Dic
【机 构】
:
Department of Parasitology,College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi
【出 处】
:
中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
The genetic variations in three mitochondrial DNA(mtDNA)regions,namely portion of cytochrome c oxidase subunit 1(pcox1),NADH dehydrogenase subunit 1(pnad1)and cytochrome b(pcytb),were examined for Dicrocoelium dendriticum samples isolated from different origins in Shaanxi province,northwestern China.The intra-specific sequence differences within D.dendriticum samples were 0-0.52%for pcox1,0-0.73%for pnad1,and 0-0.58%for pcytb.Phylogenetic analyses based on combined sequences of three mtDNA showed that all D.dendriticum samples were clustered together in same clade of Paragonimus westermani.But the phylogenetic trees could not revealed geographically genetic relationships of D.dendriticum isolates in this province.These findings will provide basic information for further study of molecular epidemiology and control ofD.dendriticum infection in this province as well as in China.
其他文献
Toll样受体是重要的模式相关分子,在树突状细胞表面有Toll样受体,其中只有TLR2和TLR4受体能够识别宿主胞外的蛋白.为探究日本血吸虫钙网质蛋白(SjCRT)究竟是通过TLR2还是TLR4途径活化宿主的树突状细胞,本研究利用TLR2-/-和TLR4-/-基因敲除的6-8周的C57BL/6J小鼠,以正常的6-8周的C57BL/6J小鼠作为对照,常规法取小鼠胫节和股骨骨髓细胞分离培养收集三组小鼠
血吸虫病是对人类健康威胁仅次于疟疾的全球第二大寄生虫病,其流行于全球78个国家和地区,至少2.49亿人和大量牲畜被感染,受威胁人口逾7亿(世界卫生组织,2014年2月).在六种可以感染人的血吸虫中,只有日本血吸虫在我国流行,但它却是所有血吸虫中生物学特性最复杂、危害最严重、防治难度最大的一种,也是唯一一种人兽共患寄生虫.吡喹酮是治疗血吸虫病的唯一特效药,但无法防止反复感染的弊端和产生抗药性的风险一
Background: The host-interactive tegument of Schistosomes appears to be a successful strategy to survival in the blood stream involving in nutrition uptake and immune evasion.The outer-tegument membra
Schistosomiasis remains a serious public health concern in China.BALB/c mice are susceptible to Schistosomajaponicum infection,whereas the Wistar rats are less susceptible to this parasite.In the curr
Introduction The primary cause of death in Schistosomaiasis is the formation of liver egg granulomas and secondary hepatic fibrosis.The pathological basis of Schistosomaiasis is the hosts immune respo
Background: Schistosomiasis is one of the worlds most prevalent zoonotic diseases and a serious worldwide public health problem.Since the tegument(TG)of Schistosoma japonicum is in direct contact with
Schistosomiasis remains a serious public health problem,with 200 million people infected and 779 million people at risk worldwide.The schistosomulum is the early stage of the complex lifecycle of Schi
摸清放牧绵羊肝片吸虫感染动态,为进一步合理、有效地防治青海省放牧绵羊肝片吸虫病提供参考。在青海省环青海湖北岸地区,选择未经驱虫与驱虫幼年羊,采用沉淀法与局部蠕虫学检查法,采用定点、定期、定量连续一年每月固定日进行剖检,每月剖检未驱虫幼龄羊5只,一年共60只,逐月统计试验羊肝片吸虫感染率、荷虫量。结果:逐月粪检与剖检结果表明,未驱虫幼龄羊肝片吸虫粪便虫卵排卵规律与剖检肝片吸虫感染率、荷虫量的动态变化
目的 建立一种能快速鉴定横川后殖吸虫和扇棘单睾吸虫的二重PCR方法.方法 从GenBank中获取横川后殖吸虫和扇棘单睾吸虫以及与其同源性较高的虫种的ITS1序列,应用Primer premier 5.0软件设计两对特异性引物,并优化PCR反应体系和反应条件,建立二重PCR法.将横川后殖吸虫和扇棘单睾吸虫与17个相关虫种一起进行PCR扩增,检测方法的特异性;并将质粒进行梯度稀释,检测其灵敏性.应用新
Liver fibrosis is a hallmark of clonorchiasis suffered by millions people in Eastern Asian countries.However,little is known to date about the expression of transforming growth factor-β(TGF-β)and othe