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Objective:To investigate the regulative effect of genistein on the apoptosis of the xenografted tumors of human ovarian carcinoma H(O) 8910PM cell on the nude mice.Method:Huma an ovarian carcinoma H(O)8910PM were cul(t)ured invitro.The models of xenografted tumor were established by the transplantation of human ovarian carcinoma H(O) 8910PM on nude mice.The nude mice were randomly divided into four groups of three treatment groups (in which genistein were a(d)ninistered ip at 5,25 and 50 mg· kg-1· d-1,respectively,for4 weeks)and one control group,the cell cycle and a(p)optosis of the xenografted tumcrs were measured by flow cytometry,the expression of bc(l) 2,Fas and FasL gene of xenografted tum(o)rs were determinod by the immunohistochemistry and the morphology of tumor cells was observed by electron m(i)croscope.Result:The tumor weights of 50 mg· kg-1 genistein group were more lighter(P＜ 0.05) compared to those of control group.The tur mot cells in G(o)G1 phase were increased,at saml time the cells in S phase were decreased (P＜ 0.01) after the treatment of 50 mg· kg-1 genistein.In addition the apoptosis rate of the cell treated with 50 mg· kg 1 genistein group was (15 14±2.27) ％,which was significantly higher than that in the control group(3.12±1.12) ％(P＜ 0 01).The expression of bc(l) 2 of the xenografted tumors was decreased and the expre(s)sion of Fas was increased in 50 rmg· kg-1 genistein group,both showing a significant difference from those in control group (P＜ 0.05).The apoptosis of the tumor cells were found more under elelectron microscopy in 50 mg· kg-1 genistein group thin those in control group.Concl(u)sion:Genistein could significantly inhibite the proliferation and induce the apoptosis of the H(O) 8910PM cell of xenografied tumors by regulating the cell cycle and apoptoic gene in the nude mice.