Purpose:In this study,we examined inhibiting angiogenic effects of Albiziajulibrissin bark extract (AJBE) in vitro and in vivo.Methods:In the presence of AJBE,the growth of human microvascular endothelial cells (HMEC-1) was evaluated by the SRB assay.According to LDH assay,we assessed which concentration of AJBE could cause the cytotoxicity.The effects of AJBE on the migration and tube formation of HMEC-1 were evaluated via migration assay and tube formation assay.Apoptosis was induced by AJBE via Hoechst staining.In vivo,Antiangiogenic effects of AJBE were assessed by planted hepatic carcinoma cells in the nude mice model and CD31 staining.Western blot analysis was used to investigate the related mechanism.Results:In vitro,IC50 value of HMEC-1 was 3.36±0.19μg/ml with the treatment of AJBE.Meanwhile,AJBE remarkably suppressed migration and tube formation of HMEC-1 at the concentration of 2.5 and 5μg/ml and induced apoptosis of HMEC-1.In addition,when LDH activity appeared to be positive,the concentration of AJBE was 40μg/ml.In vivo,AJBE inhibited tumor growth as well as the vascular density (MVD) of tumor in nude mice at the concentration of 2,4 and 8 mg/kg.Moreover,AJBE blocked the related downstream signaling molecules,including p38 MAPK and Akt with EGF induction.Conclusion:AJBE possessed antiangiogenic activities in vitro and in vivo,which could be a potent active and antiangiogenic drug.