MicroRNA-30b调控高磷诱导的血管平滑肌细胞钙化的研究

来源 :2018年中国医院协会血液净化中心管理分会年会暨第十届中国血液净化论坛、第三届亚太地区透析通路会议&2017年河南省医学 | 被引量 : 0次 | 上传用户:ijlusr
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  目的 探究miRNA-30b(miR-30b)在高磷诱导的血管平滑肌细胞(vascular smoothmuscle cells,VSMCs)钙化过程中的作用及机制。方法 选取80-100g 健康雄性SD 大鼠,采用组织贴壁法体外分离培养原代大鼠胸主动脉VSMCs,用10mM β ?甘油磷酸盐(β ?glycerophosphate,β ?GP)诱导钙化。将VSMCs随机分为3 组:正常对照组(10%DMEM),β ?GP 诱导7 天组(10%DMEM+10mM β ?GP 7d)和β ?GP 诱导14 天组(10%DMEM+10mM β ?GP 14d)。
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