利用血卟啉激光对肿瘤进行光动力学治疗,虽已有十余年历史,并已积累了许多临床和实验研究资料,但仍有许多问题有待深入。肾上腺是机体重要的内分泌器官之一,光动力学作用对肾上腺的损伤如何?迄今未见详细的研究报道。本文通过动物实验作了电镜观察。本实验采用正常NIH小鼠,腹腔注射国产扬州血卟啉,剂量为10mg/kg。24小时后于全麻下切开腹壁暴露左删肾上腺,用氦氖激光直接照射。激光波长6328A,照光强度238mW/cm~2,照光剂量100J/cm~2。照光后1、3、6、12、24、48小时分别处死,每批3只,肾上腺组织块经4％甲醛1％戊二醛混合固定后,再以1％四氧锇再固定,Epon812包埋,超薄切片经醋酸铀、柠檬酸铅双重染色,用Philips EM-410透 Although hematoporphyrin laser photodynamic therapy of tumors has been more than ten years old, and has accumulated a lot of clinical and experimental research data, but there are still many problems to be further. The adrenal gland is one of the important endocrine organs of the body, how is the photodynamic effect on adrenal damage? So far no detailed study has been reported. In this paper, animal experiments conducted electron microscopy. In this experiment, normal NIH mice were injected intraperitoneally with Yangzhou hematoporphyrin at a dose of 10 mg / kg. Twenty-four hours later, the abdominal wall was opened and the left adrenal was excised under general anesthesia. He-Ne laser was irradiated directly. Laser wavelength 6328A, light intensity 238mW / cm ~ 2, light dose 100J / cm ~ 2. 1, 3, 6, 12, 24, 48 hours after irradiation, each group was sacrificed, and 3 rats in each group. The adrenal tissue was fixed with 1% glutaraldehyde 4% formaldehyde and then re-fixed with 1% Buried, ultra-thin sections were double stained with uranyl acetate and lead citrate and see through with Philips EM-410