Separation is a critical process for both posttreatment of synthesis and sample pretreatment of instrumental analysis.While shrinking separation columns to micro-or nano-scale,separation speeds and ef
Digital microfluidics(DMF)based on electrowetting on dielectric(EWOD)has become a modern paradigm for Lab-on-a-chip(LOC)or micro-total-analysis-system(μTAS)applications,as it offers advantages of port
In this report,we are introducing our recent progress for gradient generation on Microfluidic Paper Analytical Devices(μPADs),which is a hot research frontiers of point of care test(POCTs).An abnormal
Here we present a facile sample partitioning method to enable rapid and low-cost digital PCR(dPCR)assays.This method uses the self-digitization(SD)chip platform,a microfluidic device without requireme
In the past few decades,protein-templated fluorescent gold nanoclusters have attracted much attention in the fields of bio-sensing as their excellent optical properties and good biocompatibility.In th
Efficient sample pretreatment of N-glycans from glycoproteins is essential but challenging due to the limitations of existing tedious and laborious methods in N-glycomics [1].This study aimed to estab
2017 年,人们对欧洲空间站表示密切的关注,其原因在于该机构认为飞溅下来的火箭部件中含有的肼衍生物会对生态环境造成破坏[1]。与此同时,人们考虑到肼作为一种重要的活性碱,它的广泛使用将不可避免地给环境和人类带来危害[2,3]。因此,提出了一种新的近红外荧光探针,专门用于监测封闭空间中积聚的肼蒸气和检测水样中的肼。实验表明该探针对肼有良好的响应信号和选择性,反应前后明显的颜色变化可以通过肉眼直接观
INTRODUCTION Digital polymerase chain reaction(dPCR)using fluorescence images for collecting quantitative information needs efficient software tools to automate the image analysis process.However,due
癌症的治疗已经受到广泛关注，设计和合成能够区分癌细胞的荧光探针很有必要。我们开发了一种新型的开启型荧光探针，该探针以丙烯酰基作为检测半胱氨酸的反应位点，以生物素作为靶向癌细胞的靶向单元，并使用荧光素衍生物作为荧光团。当向含有探针的体系中添加半胱氨酸时，由于半胱氨酸和丙烯酰基亲核反应释放荧光团，使得荧光发射显著增强。
均相免疫分析在蛋白质定量检测中发挥着重要作用，均相免疫不仅解决了异相免疫分析耗时耗力的问题，更降低了多次操作带来的累积错误[1]。因此均相免疫分析广泛用于多种蛋白的检测和分析中[2]。多组分均相免疫分析是在均相溶液中同时对多种蛋白进行免疫分析反应，完成快速准确的多组分定量分析[3]。