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Objective To evaluate the use of a combination of human insulin gene-modified umbilical cord mesenchymal stromal cells (hUMSCs) with silk fibroin 3D scaffolds for adipose tissue engineering.Metheds hUMSCs were isolated and cultured.HUMSCs infected with Adeinsulin-EGFP were seeded in fibroin 3D scaffolds with uniform 50-60 μm pore size.Silk fibroin scaffolds with untransfected hUMSCs were used as control.