Construction of Engineering Adipose-Like Tissue in Vivo Utilizing Human Insulin Gene-Modified Umbili

来源 :第三届全球华裔整形外科医师大会 | 被引量 : 0次 | 上传用户:Tsianyong
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Objective To evaluate the use of a combination of human insulin gene-modified umbilical cord mesenchymal stromal cells (hUMSCs) with silk fibroin 3D scaffolds for adipose tissue engineering.Metheds hUMSCs were isolated and cultured.HUMSCs infected with Adeinsulin-EGFP were seeded in fibroin 3D scaffolds with uniform 50-60 μm pore size.Silk fibroin scaffolds with untransfected hUMSCs were used as control.
其他文献
We have developed a new technique for the treatment of mild types cryptotia in which the cavum eonchae cartilage was pulled superiorly,sutured it and temporal bone to temporal parietal junction perios
背景:由于薄皮瓣既能满足美容的需要又能兼顾功能修复,故近些年来备受注目,以往我们曾经介绍过穿支型的薄皮瓣,以及远端附加血管吻合的巨大薄皮瓣,本次介绍的是在前者的基础上改进的、经扩张后的超薄穿支皮瓣,是目前最薄的一种带穿支的超薄皮瓣。非常适用于面颈部位的修复。
Objective To discuss the width frontalis muscle flap suspension in treatment of severe blepharoptosis effect.Method 52 eyes of 36 patients with severe congenital blepharoptosis were treated by width f
Background:The external ear and adjacent skin were always involved in extensive deep bum of the head and the face.Adhesion between auricle and retroauricular cranial wall and mastoidea could occur aft
Objective To explore operation effectiveness of lower eyelid blepharoplasty.Methed 208 cases were performed by invasive transconjunctival lower eyelid blepharoplasty,outside approach of ascia palpebra
Objective To investigate the effect of hypoxia on the adipogenic defferentiation potential of human umbilical cord mesenchymal stem cells (hUCMSCs),and provide experimental evidence for selecting seed
Objective:To investigate the distribution of rat bone marrow mesenchymal stem cells (BMSCs) transfected with Ad-EGFP in kidney and pancreatic tissues,and the variation of blood glucose,weight and ulce
Objective To study the capacity to construct tissue engineering adipose in vivo with human umbilical cord mesenchymal stem cells (hUCMSCs)transfected by lentiviral expression vector and silk fibroin s
Objective To study the construction of tissue engineering adipose in vitro with silk fibroin scaffold and human umbilical cord mesenchymal stem cells (hUCMSCs)transfected by recombinant human insulin
Objective to construct a recombinant adenovirus vector that containing hVEGF165 (pAdxsi-EGFP-hVEGF165),and to observe the expression of hVEGF165 by transfecting pAdxsi-EGFP-hVEGF165 into rat bone marr