【摘 要】
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Rhodococcus sp.P14 was isolated from crude oil-contaminated sediments and showed catabolic ability on various polycyclic aromatic hydrocarbons.By analyzing its whole genome sequence,a gene cluster enc
【机 构】
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Department of Biology, College of Science, Shantou University, Shantou, China
【出 处】
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第二十次全国环境微生物学学术研讨会
论文部分内容阅读
Rhodococcus sp.P14 was isolated from crude oil-contaminated sediments and showed catabolic ability on various polycyclic aromatic hydrocarbons.By analyzing its whole genome sequence,a gene cluster encoding a ring-hydroxylating dioxygenases Baa related to PAHs degradation was identified.Protein sequence analysis of the large subunit BaaA showed 64.8% identity to BenA from Acinetobacter sp.ADP1.The transcriptional level ofgene baaA was up-regulated when P 14 was cultured with pyrene,phenanthrene,anthracene or benz[a]anthracene as the sole carbon source.Further investigations on the recombinant protein Baa in E.coli demonstrated that it is able to degrade anthracene and benz[a]anthracene,resulting in 7,12-dihydroxybenz[a]anthracene and 9,10-dihydroxyanthracene as metabolites,respectively.These results indicated that Baa plays an important role in PAHs degradation in Rhodococcus sp.P14.
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