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Objective: To prepare detoxified rapeseed peptides by ultrasound-assisted enzymatic method and access sinchronic toxicity of detoxification rapeseed peptides according to the results of ninty days feeding study.Material: Rapeseed meal was purchased from Jiang Su Tianqi Biological Technology Co.,Ltd.;Alcalase,provided by Novozymes (China) Biotechnology Co.,Ltd.;Tri-frequency flat ultrasonic equipment,made by Jiangsu University.