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Aim To investigate the protection effect of the compatibility of baicalin and geniposide (7 ∶ 3) on bloodbrain barrier (BBB) damage and the mechanism of downregulating the expression of AQP4 protein incerebral ischemia reperfusion injury (CIRI) rats.Method: 100 rats were divided into 5 groups: sham, CIRI model group, baicalin and geniposide (7 ∶ 3) (30 mg · kg1 ,60 mg · kg1) group, allyl chloride (0.0021 ml · kg1)group.The model of CIRI made by improved suture method, Neural function defect, morphology and number of neurons in cerebral cortex was observed by Nissl staining; tested the contental change of Pgp and Na + , K +ATP enzymes of brain tissue, the contental change of S100β, Glucose, pyruvic acid and lactic acid of plasma by ELISA; the dry wet weight and Evans Blue (EB) tracing method served BBB permeabilitical changes; immunohistochemistry staining and semiquantitation analysis were performed to detect the AQP4 and GFAP in cerebra ischemia; the expression of AQP4 in cerebra hippocampus was determined by RTqPCR and Western blot; pathological change was observed in brain issueby HE staining; observed the changes of brain tissue uhrastructure usingtransmission electron microscope with Lanthanum nitrate tracer ; monitored the size and location of the ischemia injury in brain regions with magnetic resonance imaging (MRI).Results Compared with CIRI group, baicalin and geniposide group can restore nerve function defect; increase the number of Nissl positive cells in cerebral cortex; weaken Na + ,K+ATP enzyme dynamic, reduce the content of S100β Glucose and Pgp, reduce the content of water and volume EB in brain, elevated the7content of pyruvic acid and pyruvic acid; remarkable attenuation of AQP4 and GFAP overexpression in the brain; remarkable attenuation of AQP4 mRNA expression in hippocampus; The morphology is became clear, eased lumen of blood vessel compression deformation, loose organization, lessened cell volume and edema; Reduction of lanthanum particles into the blood vessels and the cells, reduce the vascular endothelial cell edema.Conclusion Baicalin and geniposide (7 ∶ 3) can reduce the permeability of blood brain barrier, and has a protective effect on the brain edema induced by CIRI.