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The inheritance of seed coat colour in B.napus is mainly determined by polygene.In this research,a high-density genetic linkage map was constructed with 1089 loci (456 for SSR,97 for RAPD,451 for SRAP and 75 for IBP) using the RILs,which were derived from a cross between black-seeded male parent Zhongyou 821 and yellow-seeded female parent GH06 and had been selfed for 10 successive generations by single seed propagating.