【摘 要】
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Practical live-cell super-resolution(SR)techniques are long-desired in many routine biological labs to image biomolecule dynamics.However,the current methods either require sophisticated optical setup
【出 处】
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第二届中国计算机学会生物信息学会议
论文部分内容阅读
Practical live-cell super-resolution(SR)techniques are long-desired in many routine biological labs to image biomolecule dynamics.However,the current methods either require sophisticated optical setups and deep experts or have difficulties to achieve high spatial and temporal resolutions simultaneously.Here we present a powerful single molecule guided Bayesian localization microscopy(SIMBA),which uses simple off-the-shelf total internal reflection fluorescence(TIRF)equipment to produce an appropriate 50 nm SR image with 0.5-2 s total acquisition time in living cells.The SIMBA calculates a series of whole-cell live structures for 50 time points on a desktop computer.
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