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Purpose:To discover chemoresistant-associated proteins with the resistant model cell lines.Methods:we used the isobaric tags for relative and absolute quantitation(iTRAQ) approach combined with nano liquid chromatography-tandem mass spectrometry (NanoLC-MS/MS) analysis to identify proteins that were differentially expressed between nasopharyngeal carcinoma cell lines CNE1, CNE2 and their CDDP resistance cell lines CNE1DDP and CNE2DDP.Results:By three employing biological and technical replicates, we identified 817 nonredundant proteins,64 of which were differentially expressed in the two group cell lines(CNElvs.CNE1DDP and CNE2 vs.CNE2DDP).Through the bioinformatics:Go analysis,KEGG pathway and Protein-protein Interaction of differentially expressed proteins,some proteins such as HSPB1,CLIC1,ANXA1,TPM4, Plastin-2 ,PDIA1 and so on, might play an important role in the mechanisms of chemoresistance and clinical theray.Conclusion:The present data first time show that the chemotherapy resistant protein spectrum through these 4 cell lines,and may excavate novel potential biomarkers for early detection of chemoresistance in nasopharyngeal carcinoma.