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The microbial community in large-scale digester of Hailin Farm was investigated using 454 pyrosequencing technology and PCR-DGGE.The total volume of digester was 1920 m3 and conducted in semi-continuous mode, which fed with cow manure at an OLR of 2 kg VS m-3 d-1 and HRT of 50days under mesophilic conditions (35 ℃)